dualcer

Summary about Dualcer product

Dualcer is a meticulously crafted product that has been developed in cutting-edge scientific laboratories by a team of dedicated and accomplished scientists. Our commitment to harnessing the best of science and nature is evident in every aspect of this innovative product, carefully designed to provide natural remedies for your benefit. Dualcer draws its inspiration from a remarkable botanical source with a rich historical legacy, one that has been utilized by indigenous communities for centuries as part of their traditional practices.

Extensive and rigorous scientific investigations have been carried out to thoroughly understand the composition and potential benefits of Dualcer. These investigations encompassed various tests, including chemical, biological, pharmacological, and toxicological assessments, conducted both in controlled laboratory settings (in vitro) and within living organisms (in vivo). The purpose was to uncover the primary components of the product and explore their potential efficacy.

One of the notable findings from our research is Dualcer's significant wound healing potential, demonstrating a success rate of over 95% in experimental animals. Furthermore, the product exhibited vasoconstriction effects on blood vessels after 48 hours and demonstrated anti-inflammatory properties.

In addition to its wound healing capabilities, Dualcer has shown promise as an antibacterial, antifungal, and anti-oomycete agent against a wide range of bacteria, including but not limited to Clostridium perfringens, Staphylococcus aureus, Escherichia coli, S.tritci, B.cinereae, and P.infectants.

Our product has already been successfully utilized in over 50 cases as a natural and safe topical treatment. The recommended application frequency typically ranges from 4 to 5 times per day, with the initial step of cleansing the treatment area using antiseptic solutions to ensure cleanliness. The duration of treatment varies based on the severity of the condition, ranging from weeks to several months.

These collective findings underscore Dualcer's remarkable safety profile and its potential as a therapeutic topical agent, providing confidence in its practical application for various health-related needs.

Summary of the Invention

  1. Chemical analysis using both HPLC and Spectrophotometer indicated that the extract of the product DuAlcer contains significant amounts of phenolic and flavonoids.
  2. The extract of the product DuAlcer showed potential healing effect using scratch and transwell migration assay.
  3. The wound healing effect after 72 hours of treatment with the extract with 0.3 & 0.03 mg/ml of the extract of the product exhibited complete closure.
  4. The extract's inflammatory response was investigated and it was found that the secretion levels of IL-1 beta, IL-6, TNF-alpha, IL-8 and IL-20p70 were upregulated and IL-10 was down regulated significantly compared to the negative control.
  5. The extract of the product showed no cytotoxic effect even at concentrations more than 30 mg/ml. Additionally, the extract's inflammation response was evaluated using human monocytic cell line THP-1 and the secretion of the inflammatory cytokines were measured using CBA array beads. The IC50 value for the extract was 9.799 mg/ml.
  6. The acute dermal toxicity of the extract study plan exceeds the requirements of the following internationally accepted guidelines and recommendations: OECD Guidelines for testing of chemicals, Section 4, No. 402 "Acute Dermal Toxicity", Adopted 9 October 2017. In conclusion, the results showed:
    • There were no mortality or morbidity occurred during the experiment period.
    • Animals did not show any signs and symptoms related to dermal toxicity such as changes in skin and fur (erythema, oedema, corrosion, itching etc.), eyes and mucous membranes, and also respiratory, circulatory, autonomic and central nervous systems, and somatomotor activity and behavior pattern following the application of test item at 30 min, 1, 2, 4 and 24 h and till day 14.
    • Body weight gain was found to be normal in the test item applied animals.
  7. In addition, the oral acute toxicity of the extract was performed according to the OECD guideline 425 (Acute Oral Toxicity - up and down procedure) adopted on 16 October 2008.
  8. The LD50 value was found to be greater than 2 gm/kg body weight and classified as Category-5 based on Globally Harmonised Classification System (GHS) for Chemical Substances and Mixtures.
  9. Therefore, the extract of the product is safe and, as a result, it was formulated as either cream or ointment semi-solid dosage forms.
  10. Indications:
    • Sepsis and dead tissues
    • First- & second-degree burns
    • Severe bacterial infection
    • Chronic wounds

Important Notes

  1. This product is 100% natural. The phytochemical composition of the extract was completely investigated using many up-to-date techniques. These data were published in high impact journals - Scopus indexed.
  2. The extract of the invention was tested on more than 30 patients to see if it causes any allergic reactions.
  3. The previous test was performed by Dermatest, a leading independent dermatological testing institute founded in Münster, Germany, in 1978. It evaluates the safety and efficacy of cosmetics, supplements, and consumer goods. The company rigorously tests products on human volunteers to ensure they are skin-friendly and awards an internationally recognized seal of approval.
  4. Clinically, the product was tested on more than 70 patients (terminal cases) suffering from gangrene and the results showed that its potential effect could reach more than 85% success.